rabbit anti-thy1 polyclonal antibody h-110 Search Results


anti thy 1 h 110  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti thy 1 h 110
Anti Thy 1 H 110, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tma slides  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tma slides
Figure 3 Immunohistochemical staining <t>of</t> <t>THY1</t> in NPC <t>TMA</t> tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes
Tma Slides, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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igg antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology igg antibody
Figure 3 Immunohistochemical staining <t>of</t> <t>THY1</t> in NPC <t>TMA</t> tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes
Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti actin  (Valiant Co Ltd)
96
Valiant Co Ltd mouse anti actin
Figure 3 Immunohistochemical staining <t>of</t> <t>THY1</t> in NPC <t>TMA</t> tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes
Mouse Anti Actin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-thy1+polyclonal+antibody+h-110/Actin/pmc05829086-52-11-15
Average 96 stars, based on 1 article reviews
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gata4 c 20  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology gata4 c 20
Figure 3 Immunohistochemical staining <t>of</t> <t>THY1</t> in NPC <t>TMA</t> tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes
Gata4 C 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-thy1+polyclonal+antibody+h-110/GATA-4+Antibody/pmc05829086-52-29-31
Average 97 stars, based on 1 article reviews
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n-cadherin  (Becton Dickinson)
90
Becton Dickinson n-cadherin
Figure 3 Immunohistochemical staining <t>of</t> <t>THY1</t> in NPC <t>TMA</t> tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes
N Cadherin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-thy1+polyclonal+antibody+h-110/n+cadherin+antibody/pmc05829086-52-17-18
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lc3b  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc lc3b
(A) Senescence-associated beta-galactosidase staining in control and cis/pem adapted primary cultures from patient malignant pleural mesothelioma (MPM) 236 and MPM95. Scale bar 25 µm. (B) Expression of senescence-associated secretion phenotype-associated GATA-4 and autophagy activation <t>LC3B</t> and p62. (C) DNA-damage response markers γ-H2AX and p53 stabilization.
Lc3b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mesothelin  (Rockland Immunochemicals)
93
Rockland Immunochemicals mesothelin
(A) Senescence-associated beta-galactosidase staining in control and cis/pem adapted primary cultures from patient malignant pleural mesothelioma (MPM) 236 and MPM95. Scale bar 25 µm. (B) Expression of senescence-associated secretion phenotype-associated GATA-4 and autophagy activation <t>LC3B</t> and p62. (C) DNA-damage response markers γ-H2AX and p53 stabilization.
Mesothelin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-thy1+polyclonal+antibody+h-110/MESOTHELIN+ANTIBODY/pmc05829086-52-25-26
Average 93 stars, based on 1 article reviews
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mesothelin  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mesothelin
(A) Senescence-associated beta-galactosidase staining in control and cis/pem adapted primary cultures from patient malignant pleural mesothelioma (MPM) 236 and MPM95. Scale bar 25 µm. (B) Expression of senescence-associated secretion phenotype-associated GATA-4 and autophagy activation <t>LC3B</t> and p62. (C) DNA-damage response markers γ-H2AX and p53 stabilization.
Mesothelin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p21  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology p21
Inhibition of β‐catenin in FLC organoids reduces expression of FLC‐specific CEGR/ALCD‐containing genes and inhibits the development of FLC. (A) Western blot shows levels of DNAJB1‐PKAc in transfected cells. Bar graphs show luciferase activity of CEGR/ALCD‐luciferase constructs with CEGRs/ALCDs from p53, β‐catenin, and NRF2 genes in untreated cells, and in cells transfected with DNAJB1‐PKAc, untreated and treated with the inhibitor of β‐catenin PRI‐724 (5 μm). (B) Left: Treatment of FLC organoids with PRI‐724 (5 μm) for 24 h inhibits cyclin D1 and β‐catenin, but elevates <t>p21</t> and Rb. Co‐IP study shows that PRI‐724 destroys β‐catenin‐TCF4‐p300 complexes in FLC organoids. Images on the right show levels of proteins, the genes of which contain CEGRs/ALCDs. (C) Treatment of FLC organoids with PRI‐724 (5 μm) for 96 h inhibits the proliferation of organoids and the formation of organoid structures. (D) List of top up‐regulated mRNAs that were determined by RNA‐seq in FLC organoids after treatments with PRI‐724 for 96 h (5 μm, n = 4/group). (E) List of genes reduced in FLC organoids with inhibited β‐catenin activity. (F) Western blot confirms changes of expression of proteins, the mRNAs of which were found by RNA‐seq.
P21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col3a1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc col3a1
Inhibition of β‐catenin in FLC organoids reduces expression of FLC‐specific CEGR/ALCD‐containing genes and inhibits the development of FLC. (A) Western blot shows levels of DNAJB1‐PKAc in transfected cells. Bar graphs show luciferase activity of CEGR/ALCD‐luciferase constructs with CEGRs/ALCDs from p53, β‐catenin, and NRF2 genes in untreated cells, and in cells transfected with DNAJB1‐PKAc, untreated and treated with the inhibitor of β‐catenin PRI‐724 (5 μm). (B) Left: Treatment of FLC organoids with PRI‐724 (5 μm) for 24 h inhibits cyclin D1 and β‐catenin, but elevates <t>p21</t> and Rb. Co‐IP study shows that PRI‐724 destroys β‐catenin‐TCF4‐p300 complexes in FLC organoids. Images on the right show levels of proteins, the genes of which contain CEGRs/ALCDs. (C) Treatment of FLC organoids with PRI‐724 (5 μm) for 96 h inhibits the proliferation of organoids and the formation of organoid structures. (D) List of top up‐regulated mRNAs that were determined by RNA‐seq in FLC organoids after treatments with PRI‐724 for 96 h (5 μm, n = 4/group). (E) List of genes reduced in FLC organoids with inhibited β‐catenin activity. (F) Western blot confirms changes of expression of proteins, the mRNAs of which were found by RNA‐seq.
Col3a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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e2f1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology e2f1
Inhibition of β‐catenin in FLC organoids reduces expression of FLC‐specific CEGR/ALCD‐containing genes and inhibits the development of FLC. (A) Western blot shows levels of DNAJB1‐PKAc in transfected cells. Bar graphs show luciferase activity of CEGR/ALCD‐luciferase constructs with CEGRs/ALCDs from p53, β‐catenin, and NRF2 genes in untreated cells, and in cells transfected with DNAJB1‐PKAc, untreated and treated with the inhibitor of β‐catenin PRI‐724 (5 μm). (B) Left: Treatment of FLC organoids with PRI‐724 (5 μm) for 24 h inhibits cyclin D1 and β‐catenin, but elevates <t>p21</t> and Rb. Co‐IP study shows that PRI‐724 destroys β‐catenin‐TCF4‐p300 complexes in FLC organoids. Images on the right show levels of proteins, the genes of which contain CEGRs/ALCDs. (C) Treatment of FLC organoids with PRI‐724 (5 μm) for 96 h inhibits the proliferation of organoids and the formation of organoid structures. (D) List of top up‐regulated mRNAs that were determined by RNA‐seq in FLC organoids after treatments with PRI‐724 for 96 h (5 μm, n = 4/group). (E) List of genes reduced in FLC organoids with inhibited β‐catenin activity. (F) Western blot confirms changes of expression of proteins, the mRNAs of which were found by RNA‐seq.
E2f1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3 Immunohistochemical staining of THY1 in NPC TMA tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes

Journal: Oncogene

Article Title: THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma.

doi: 10.1038/sj.onc.1208812

Figure Lengend Snippet: Figure 3 Immunohistochemical staining of THY1 in NPC TMA tissues (a–d). (a and b) Normal expression of THY1 with a staining index more than 6 was observed in 2 primary NPC specimens. (c) A lymph node metastatic NPC showed downregulation of THY1 expression with a staining index less than 6. (d) Downregulated expression of THY1 was observed in another lymph node metastatic NPC, as compared to its adjacent normal lymphocytes

Article Snippet: The TMA slides were incubated with rabbit anti-THY1 polyclonal antibody (H-110, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 500 dilution) overnight.

Techniques: Immunohistochemical staining, Staining, Expressing

(A) Senescence-associated beta-galactosidase staining in control and cis/pem adapted primary cultures from patient malignant pleural mesothelioma (MPM) 236 and MPM95. Scale bar 25 µm. (B) Expression of senescence-associated secretion phenotype-associated GATA-4 and autophagy activation LC3B and p62. (C) DNA-damage response markers γ-H2AX and p53 stabilization.

Journal: Frontiers in Oncology

Article Title: Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression

doi: 10.3389/fonc.2018.00040

Figure Lengend Snippet: (A) Senescence-associated beta-galactosidase staining in control and cis/pem adapted primary cultures from patient malignant pleural mesothelioma (MPM) 236 and MPM95. Scale bar 25 µm. (B) Expression of senescence-associated secretion phenotype-associated GATA-4 and autophagy activation LC3B and p62. (C) DNA-damage response markers γ-H2AX and p53 stabilization.

Article Snippet: Membranes were probed with the following primary antibodies: anti-calretinin (Sigma, HPA007306), mouse anti-actin (#69100) from MP Biomedicals, N-Cadherin (BD Biosciences, 610920), YAP (Cell Signaling 4912), Mesothelin (Rockland Inc. 200-301-A88), GATA4 (C-20) (Santa Cruz sc-1237), p62 (Progen GP62-C), LC3B (Cell Signaling 2775S), p53 (DO-1) (Santa Cruz sc-126), Thy1 (H-110) (Santa Cruz sc-9163), and γ-H2AX (Millipore 05-636).

Techniques: Staining, Control, Expressing, Activation Assay

Inhibition of β‐catenin in FLC organoids reduces expression of FLC‐specific CEGR/ALCD‐containing genes and inhibits the development of FLC. (A) Western blot shows levels of DNAJB1‐PKAc in transfected cells. Bar graphs show luciferase activity of CEGR/ALCD‐luciferase constructs with CEGRs/ALCDs from p53, β‐catenin, and NRF2 genes in untreated cells, and in cells transfected with DNAJB1‐PKAc, untreated and treated with the inhibitor of β‐catenin PRI‐724 (5 μm). (B) Left: Treatment of FLC organoids with PRI‐724 (5 μm) for 24 h inhibits cyclin D1 and β‐catenin, but elevates p21 and Rb. Co‐IP study shows that PRI‐724 destroys β‐catenin‐TCF4‐p300 complexes in FLC organoids. Images on the right show levels of proteins, the genes of which contain CEGRs/ALCDs. (C) Treatment of FLC organoids with PRI‐724 (5 μm) for 96 h inhibits the proliferation of organoids and the formation of organoid structures. (D) List of top up‐regulated mRNAs that were determined by RNA‐seq in FLC organoids after treatments with PRI‐724 for 96 h (5 μm, n = 4/group). (E) List of genes reduced in FLC organoids with inhibited β‐catenin activity. (F) Western blot confirms changes of expression of proteins, the mRNAs of which were found by RNA‐seq.

Journal: Hepatology Communications

Article Title: β‐catenin cancer–enhancing genomic regions axis is involved in the development of fibrolamellar hepatocellular carcinoma

doi: 10.1002/hep4.2055

Figure Lengend Snippet: Inhibition of β‐catenin in FLC organoids reduces expression of FLC‐specific CEGR/ALCD‐containing genes and inhibits the development of FLC. (A) Western blot shows levels of DNAJB1‐PKAc in transfected cells. Bar graphs show luciferase activity of CEGR/ALCD‐luciferase constructs with CEGRs/ALCDs from p53, β‐catenin, and NRF2 genes in untreated cells, and in cells transfected with DNAJB1‐PKAc, untreated and treated with the inhibitor of β‐catenin PRI‐724 (5 μm). (B) Left: Treatment of FLC organoids with PRI‐724 (5 μm) for 24 h inhibits cyclin D1 and β‐catenin, but elevates p21 and Rb. Co‐IP study shows that PRI‐724 destroys β‐catenin‐TCF4‐p300 complexes in FLC organoids. Images on the right show levels of proteins, the genes of which contain CEGRs/ALCDs. (C) Treatment of FLC organoids with PRI‐724 (5 μm) for 96 h inhibits the proliferation of organoids and the formation of organoid structures. (D) List of top up‐regulated mRNAs that were determined by RNA‐seq in FLC organoids after treatments with PRI‐724 for 96 h (5 μm, n = 4/group). (E) List of genes reduced in FLC organoids with inhibited β‐catenin activity. (F) Western blot confirms changes of expression of proteins, the mRNAs of which were found by RNA‐seq.

Article Snippet: List of antibodies used in this study : ph‐S675‐β‐catenin (Cell Signaling Tech; #D2F1),TCF4 (Cell Signaling; #C48H11), p300 (Invitrogen; sc: PAI‐848), Col3A1 (Cell Signaling Tech; #30565), secreted protein acidic and cysteine rich (SPARC) (Abcam; ab203284), Thy1 (Santa Cruz; sc: H‐110), versican (VCAN) (Invitrogen; MA5‐34654), HDAC1 (EDM Millipore Corp.; 2E10), DNAJB1 (Abcam; ab69402), E2F1 (Santa Cruz; sc:C20), p21 (Santa Cruz; sc: F5), PGAP1 (Invitrogen; sc‐PA5 72,340), RUN domain‐containing 1 (RUNDC1) (Abcam; sc‐AB 151583), HECT‐type E3 ubiquitin transferase (HACE1) (Abcam; sc‐133,637), cyclin D1 (Santa Cruz; sc‐753), cdc2 (Santa Cruz; sc‐954), CYP3A4 (Santa Cruz; sc‐30,621), β‐actin (Sigma; A5316), ph‐Ser33/37/Thr41‐β‐catenin (Cell Signaling; #8561), and ph‐Tyr333‐β‐catenin (Bioss; #12856R‐CY3).

Techniques: Inhibition, Expressing, Western Blot, Transfection, Luciferase, Activity Assay, Construct, Co-Immunoprecipitation Assay, RNA Sequencing